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Drvvt test - lab report example

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DRVVT test

DRVVT test Lab Report Questions Immunological method for measuring lupus anticoagulants Activated Partial Thromboplastin Time (aPTT) is an immunological method for measuring lupus anticoagulants. In this method, reagents consisting of a platelet phospholipid, an activator, and calcium are added to a sample containing platelet poor plasma and then the time taken to form a clot is recorded. The aPTT is usually the duration it takes from when calcium is added to the formation of a clot. The nature of antibody is an Antiphospholipid antibody.
2. Lack of positive antibodies in sample C
The absence of positive antibodies in sample C means that lupus anticoagulants were not present in the blood thus blood clotting could not occur. This means that there is the presence of common pathway clotting factors in at least 30% activity.
3. Test for 2. Above
Testing for the absence of anticoagulants will result in immediate clotting is a sample of blood using the method of aPTT.
4. Mishandling patient samples
Failure to detect APS could be as a result of mishandling of patient samples. In this case, there could be an introduction of protein contaminants of the same nature thus make it difficult to distinguish APS proteins from the other protein contaminants.
5. Other tests include:
The Activated Partial Thromboplastin Time (aPTT), the Platelet Neutralization Procedure (PNP), and the Kaolin Clotting Time (KCT)
6. Anionic phospholipids
Anionic phospholipids serve as strong cofactors required for the gathering of 3 different coagulation complexes when they get exposed on the apical cell membrane surface. These coagulation complexes are the tissue factor-VIIa complex, the Xa-Va complex, and the IXa-VIIIa complex, which participate in blood coagulation. In this way, they are able to enhance the activity of phospholipid-dependent coagulation factors.
7. How vitamin K is involved in coagulation.
Vitamin K is an essential contributor in the synthesis of a number of proteins that participate in blood coagulation and also anticoagulation. It is an essential cofactor for a carboxylase enzyme that catalyses the carboxylation of glutamic acid residues found on vitamin K-dependent proteins that participate coagulation. These proteins include prothrombin factors II, VII, IX and X
Sources of vitamin K include leafy greens, liver, and cauliflower. Vitamin K is also largely synthesized in the large intestines by bacteria. It is important to note that vitamin K is fat-soluble in nature and that obtained from foods as well as that produce by microbes in the large intestines get absorbed into intestinal lymph together with other lipids.

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